What are the methods for producing lipase?

1. Microbial fermentation method
Screen microbial strains with high lipase production ability, such as bacteria, fungi, etc.
Ferment these strains in a suitable culture medium.
Promote the secretion of lipase by microorganisms in large quantities by controlling culture conditions, such as temperature, pH value, dissolved oxygen, etc.
2. Genetic engineering method
Clone the lipase gene from a lipase-producing microorganism.
Introduce the gene into a suitable host cell, such as Escherichia coli, yeast, etc.
By optimizing gene expression conditions, the host cell synthesizes lipase in large quantities.
3. Plant and animal extraction method
Extract lipase from certain plant and animal tissues rich in lipase. However, this method is usually limited by the source of raw materials and extraction efficiency.