Things to note during the extraction process of papain

1. Industries such as food and tanning that do not have high requirements for enzyme purity are suitable for tannin precipitation. The process is relatively simple, the consumption of raw materials is small, and the equipment required is simple. However, the enzyme recovery rate is relatively low and the enzyme purity is not high enough. Cosmetic ingredients and some medical and health products. It is suitable for obtaining higher purity papain after salting out, crystallization and recrystallization. In order to obtain higher purity enzymes for scientific experiments and medical and health care, the above method should be combined with shaving elution method, organic solvent precipitation method or ultrafiltration concentration method. However, these methods are relatively complex, require high quality of workers, and require large one-time investment in equipment.
2. Papain is a biologically active substance that is susceptible to inactivation caused by heavy metal ions, such as iron, copper, etc., or chemicals. Therefore, the production process requires low temperature, short time, hygiene, and avoids the effects of alkali and strong acid. .
3. The tannin precipitate produced by papain during the production process can be vacuum dried at 60°C and crushed to obtain crude enzyme. It is suitable for industries such as food and tanning that do not require high enzyme purity; after elution, the enzyme It has high strength and is suitable for scientific undertakings, as a cosmetic ingredient or in the medical and health industry.
4. Papain is a hydrolytic enzyme that stimulates and decomposes human skin. Therefore, production personnel must wear work clothes, work caps, masks, gloves, rubber shoes and other health protective equipment to do a good job in production protection.
5. Papain is a colloidal substance, and bubbles are easily generated during the production process, causing losses.