Introduction to immobilized lipase

Immobilized lipase is achieved by immobilizing or confining lipase (an enzyme that can catalyze the hydrolysis of ester bonds) in a certain space to facilitate reuse and improve the stability and efficiency of its industrial applications. Immobilized lipase is widely used in food, cosmetics, pharmaceuticals and other fields. Here are some key points about immobilized lipase:
1. Immobilization methods: including physical adsorption, covalent bonding, embedding (such as in gel or polymer matrix) and cross-linking.
2. Carrier materials: There are various carrier materials for immobilized lipase, including nanomaterials, metal organic frameworks (MOFs), porous organic polymers, porous ceramics, macroporous resins, and chitosan.
3. Advantages: Immobilization can improve the stability of lipase, enabling it to work in a wide temperature and pH range, while reducing enzyme inactivation and degradation, facilitating separation and recovery from the reaction system, and improving reusability.
4. Challenges: Challenges that may be encountered during the immobilization process include the loss of enzyme activity, low immobilization efficiency, high cost, and the stability and reusability of the immobilized enzyme.
5. Application: Immobilized lipase serves as a biocatalyst in esterification, transesterification, hydrolysis and other reactions, and is especially suitable for chiral drug synthesis, biodiesel production and flavor enhancement in the food industry.
6. Research progress: In recent years, researchers have made significant progress in the carrier materials, immobilization technology and applications of immobilized lipase, especially in the use of porous materials such as MOFs and COFs as carriers.
7. Future prospects: Future research may focus on developing more economical, efficient and stable immobilization systems, as well as exploring the application of immobilized lipase in new fields.