Uses of bioethanolase

1. Cellulase: In the production of bioethanol, cellulase is responsible for breaking down the cellulose in the biomass into glucose, which is the main substrate of the fermentation process.
2. Hemicellulase: This type of enzyme breaks down hemicellulose into five-carbon sugars such as xylose, which can then be fermented to produce ethanol.
3. Alpha-amylase: Alpha-amylase hydrolyzes starch into dextrin and maltose, providing a substrate for glucosidase.
4. Glucosidase: further hydrolyzes dextrin and maltose into glucose, increasing the amount of sugar available during fermentation.
5. Alcohol dehydrogenase (ADH): During ethanol fermentation, ADH converts acetaldehyde into ethanol, which is a key step in ethanol production.
6. Aldehyde dehydrogenase (ALDH): ALDH oxidizes ethanol to acetaldehyde during ethanol metabolism, which is the reverse process of ethanol metabolism.
7. Transglycosidase: In some cases, transglycosidase can be used to transfer sugars to other substrates to generate new compounds.
8. Bifunctional acetaldehyde/alcohol dehydrogenase AdhE: AdhE has the catalytic activity of acetaldehyde dehydrogenase and alcohol dehydrogenase, and is one of the key enzymes in the bacterial ethanol anaerobic fermentation pathway.
9. Peroxisomal catalase and aldehyde (AOX): plays a role in the oxidative biotransformation pathway of ethanol.
10. Phospholipase D (PLD): In the non-oxidative pathway of ethanol bioconversion, it participates in the re-esterification process of phospholipids.
11. Cellulosic biogasoline production: In cellulosic biogasoline production technology, enzyme hydrolysis-fermentation technology can convert raw materials such as corn stalks, corncobs and switchgrass into cellulosic ethanol.
12. Metabolic engineering: Transforming Escherichia coli through metabolic engineering strategies, using ethanol as a substrate to synthesize important chemicals such as isopropanol and 3-hydroxypropionic acid, proving the great potential of the ethanol biorefinery platform based on syngas sources.