What are the extraction methods of saccharin sodium?
Mar 30, 2024
1. Ether extraction method: This is a traditional extraction method that uses ether to extract saccharin sodium in food under acidic conditions, and then performs qualitative and semi-quantitative determination after separation and color development by thin layer chromatography.
2. Ultrasound-assisted extraction: This method uses the mechanical vibration and cavitation effect generated by ultrasound to accelerate the release of target compounds from the sample matrix and improve extraction efficiency.
3. Solid-phase extraction method: Using a solid-phase extraction column (such as HLB or C18 solid-phase extraction cartridge) to extract saccharin sodium can effectively remove impurities such as protein and fat in the sample and improve the purity of the extract.
4. Dialysis method: For samples with high protein and fat content, dialysis method can be used to allow saccharin sodium with a smaller molecular weight to penetrate into the solution to eliminate the interference of protein, starch, fat, etc.
5. Liquid chromatography: This is a commonly used analysis method. After separation by liquid chromatography, it is detected by ultraviolet detector and quantified by external standard method.
6. Gas chromatography: For properly derivatized saccharin sodium, gas chromatography can be used for analysis. This method has high sensitivity and high separation efficiency.
7. Ultra-high performance liquid chromatography tandem mass spectrometry: This is a highly sensitive and highly selective analytical method suitable for the simultaneous detection of multiple sweeteners, including saccharin sodium.
8. Nessler colorimetry: A yellow substance is generated by reacting with Nessler's reagent, and the color is quantitatively compared with the standard.
9. High performance liquid chromatography: suitable for the determination of saccharin sodium in food, with high sensitivity and accuracy.
10. Standard solution preparation method: Before chromatographic analysis, a series of concentration standard solutions are usually prepared to draw a standard curve to quantitatively analyze the saccharin sodium in the sample.
